Fast and Sensitive DNA Hybridization Through Micro-Fluidics

The research target for DNA hybridization through microfluidics is to achieve a fast and very sensitive hybridization reaction by investigating the effect of different pertinent parameters using computational method. The mathematical model that we developed couples mass transport of DNA from the bulk of the solution with the surface reaction kinetics for the heterogeneous DNA Hybridization reaction in a micro fluidic channel. A two-dimensional analysis of the classical mass transport equation under laminar flow conditions combined with an overall second order rate constant for the surface hybridization reaction elucidates the effect of changing the fluid flow rates, bulk concentration and channel heights on the DNA hybridization efficiency. The model enables the DNA researcher to optimize his DNA assays for speed and lower limit of detection.